I have the exact same problem, I haven't added anything, neither updated the vm or unraid itself.
My usb sound card (Xonar U7 MKII) suddenly didn't get recognised anymore, windows threw an error.
So after doing everything I could in the vm (reinstall drivers, troubleshoot etc) I decided to restart Unraid.
After restarting, I noticed my VM didn't autostart, while that setting is enabled and it normally should've booted.
When I manually start my vm, I get the exact error @Keenano had:
"unsupported configuration: host doesn't support VFIO PCI passthrough"
All the help would be appreciated :))
(btw, is there any way to find more info as of what could've gone wrong? UD logs provide nothing, and the VM logs only give this warning: shutting down, reason=failed)
I've just found out that the problem seems to be my VFIO-PCI plugin, which doesn't show my devices at all anymore
Also, if it can be of any help, I have PCIe ACS override enabled, both multifunction and downstream, without it, I can't passthrough even half the devices I need. This has never been an issue before tho so I doubt this has to be changed.