2 GPU's Passthrough - 2 VM's doesnt work?

Hi,

i have an problem. I have two GPU's, a GTX 970 and a GTX960, and i want to assign each to a VM.

I gave VM1 my GTX 970, and VM2 the GTX 960, VM1 runs successfully but VM2 wont start. It say's that my GTX 970 is already in use.

I looked at my IOMMU Groups and all GPUs are in the same Group.

Thats the error message: Requested operation is not valid: PCI device 0000:01:00.0 is in use by driver QEMU, domain Windows 10

Thats my PCI list:

00:00.0 Host bridge [0600]: Intel Corporation 4th Gen Core Processor DRAM Controller [8086:0c00] (rev 06)
00:01.0 PCI bridge [0604]: Intel Corporation Xeon E3-1200 v3/4th Gen Core Processor PCI Express x16 Controller [8086:0c01] (rev 06)
00:01.1 PCI bridge [0604]: Intel Corporation Xeon E3-1200 v3/4th Gen Core Processor PCI Express x8 Controller [8086:0c05] (rev 06)
00:02.0 VGA compatible controller [0300]: Intel Corporation Xeon E3-1200 v3/4th Gen Core Processor Integrated Graphics Controller [8086:0412] (rev 06)
00:03.0 Audio device [0403]: Intel Corporation Xeon E3-1200 v3/4th Gen Core Processor HD Audio Controller [8086:0c0c] (rev 06)
00:14.0 USB controller [0c03]: Intel Corporation 9 Series Chipset Family USB xHCI Controller [8086:8cb1]
00:16.0 Communication controller [0780]: Intel Corporation 9 Series Chipset Family ME Interface #1 [8086:8cba]
00:19.0 Ethernet controller [0200]: Intel Corporation Ethernet Connection (2) I218-V [8086:15a1]
00:1a.0 USB controller [0c03]: Intel Corporation 9 Series Chipset Family USB EHCI Controller #2 [8086:8cad]
00:1b.0 Audio device [0403]: Intel Corporation 9 Series Chipset Family HD Audio Controller [8086:8ca0]
00:1c.0 PCI bridge [0604]: Intel Corporation 9 Series Chipset Family PCI Express Root Port 1 [8086:8c90] (rev d0)
00:1c.3 PCI bridge [0604]: Intel Corporation 9 Series Chipset Family PCI Express Root Port 4 [8086:8c96] (rev d0)
00:1c.4 PCI bridge [0604]: Intel Corporation 9 Series Chipset Family PCI Express Root Port 5 [8086:8c98] (rev d0)
00:1c.6 PCI bridge [0604]: Intel Corporation 9 Series Chipset Family PCI Express Root Port 7 [8086:8c9c] (rev d0)
00:1d.0 USB controller [0c03]: Intel Corporation 9 Series Chipset Family USB EHCI Controller #1 [8086:8ca6]
00:1f.0 ISA bridge [0601]: Intel Corporation 9 Series Chipset Family Z97 LPC Controller [8086:8cc4]
00:1f.2 SATA controller [0106]: Intel Corporation 9 Series Chipset Family SATA Controller [AHCI Mode] [8086:8c82]
00:1f.3 SMBus [0c05]: Intel Corporation 9 Series Chipset Family SMBus Controller [8086:8ca2]
01:00.0 VGA compatible controller [0300]: NVIDIA Corporation GM204 [GeForce GTX 970] [10de:13c2] (rev a1)
01:00.1 Audio device [0403]: NVIDIA Corporation GM204 High Definition Audio Controller [10de:0fbb] (rev a1)
02:00.0 VGA compatible controller [0300]: NVIDIA Corporation GM206 [GeForce GTX 960] [10de:1401] (rev a1)
02:00.1 Audio device [0403]: NVIDIA Corporation Device [10de:0fba] (rev a1)
04:00.0 PCI bridge [0604]: ASMedia Technology Inc. Device [1b21:1187]
05:01.0 PCI bridge [0604]: ASMedia Technology Inc. Device [1b21:1187]
05:02.0 PCI bridge [0604]: ASMedia Technology Inc. Device [1b21:1187]
05:03.0 PCI bridge [0604]: ASMedia Technology Inc. Device [1b21:1187]
05:04.0 PCI bridge [0604]: ASMedia Technology Inc. Device [1b21:1187]
05:05.0 PCI bridge [0604]: ASMedia Technology Inc. Device [1b21:1187]
05:06.0 PCI bridge [0604]: ASMedia Technology Inc. Device [1b21:1187]
05:07.0 PCI bridge [0604]: ASMedia Technology Inc. Device [1b21:1187]
06:00.0 Audio device [0403]: Creative Labs Sound Core3D [sound Blaster Recon3D / Z-Series] [1102:0012] (rev 01)
07:00.0 Network controller [0280]: Broadcom Limited BCM4352 802.11ac Wireless Network Adapter [14e4:43b1] (rev 03)
08:00.0 SATA controller [0106]: ASMedia Technology Inc. ASM1062 Serial ATA Controller [1b21:0612] (rev 02)
0b:00.0 Ethernet controller [0200]: Intel Corporation I211 Gigabit Network Connection [8086:1539] (rev 03)
0d:00.0 SATA controller [0106]: ASMedia Technology Inc. ASM1062 Serial ATA Controller [1b21:0612] (rev 02)
0e:00.0 USB controller [0c03]: ASMedia Technology Inc. ASM1042A USB 3.0 Host Controller [1b21:1142]

Thats my IMMOU Group list:

/sys/kernel/iommu_groups/0/devices/0000:00:00.0
/sys/kernel/iommu_groups/1/devices/0000:00:01.0
/sys/kernel/iommu_groups/1/devices/0000:00:01.1
/sys/kernel/iommu_groups/1/devices/0000:01:00.0
/sys/kernel/iommu_groups/1/devices/0000:01:00.1
/sys/kernel/iommu_groups/1/devices/0000:02:00.0
/sys/kernel/iommu_groups/1/devices/0000:02:00.1
/sys/kernel/iommu_groups/2/devices/0000:00:02.0
/sys/kernel/iommu_groups/3/devices/0000:00:03.0
/sys/kernel/iommu_groups/4/devices/0000:00:14.0
/sys/kernel/iommu_groups/5/devices/0000:00:16.0
/sys/kernel/iommu_groups/6/devices/0000:00:19.0
/sys/kernel/iommu_groups/7/devices/0000:00:1a.0
/sys/kernel/iommu_groups/8/devices/0000:00:1b.0
/sys/kernel/iommu_groups/9/devices/0000:00:1c.0
/sys/kernel/iommu_groups/10/devices/0000:00:1c.3
/sys/kernel/iommu_groups/11/devices/0000:00:1c.4
/sys/kernel/iommu_groups/12/devices/0000:00:1c.6
/sys/kernel/iommu_groups/13/devices/0000:00:1d.0
/sys/kernel/iommu_groups/14/devices/0000:00:1f.0
/sys/kernel/iommu_groups/14/devices/0000:00:1f.2
/sys/kernel/iommu_groups/14/devices/0000:00:1f.3
/sys/kernel/iommu_groups/15/devices/0000:04:00.0
/sys/kernel/iommu_groups/16/devices/0000:05:01.0
/sys/kernel/iommu_groups/16/devices/0000:06:00.0
/sys/kernel/iommu_groups/17/devices/0000:05:02.0
/sys/kernel/iommu_groups/17/devices/0000:07:00.0
/sys/kernel/iommu_groups/18/devices/0000:05:03.0
/sys/kernel/iommu_groups/18/devices/0000:08:00.0
/sys/kernel/iommu_groups/19/devices/0000:05:04.0
/sys/kernel/iommu_groups/20/devices/0000:05:05.0
/sys/kernel/iommu_groups/21/devices/0000:05:06.0
/sys/kernel/iommu_groups/21/devices/0000:0b:00.0
/sys/kernel/iommu_groups/22/devices/0000:05:07.0
/sys/kernel/iommu_groups/23/devices/0000:0d:00.0
/sys/kernel/iommu_groups/24/devices/0000:0e:00.0

EDIT: ACS override is enabled

EDIT 2: ACS override was not correctly enabled, i just add an param to the syslinux.cfg and now i can start both GPUs but my Windows vm doesnt show anything

EDIT 3: I plugged my GPU into my Third PCIE Slot, got now only PCIE Gen2 x2, but i think its okay, needs some testing.