Thanks. To clarify, when using the GUI to initiate a transcode I can and have unchecked that box to remove the default tag. When I do this the file works properly and no subtitles display by default in playback. However, I am setting this up to be automated through watch folders and the preset I have created does not maintain that subtitle status. Anytime I load the preset it checks the default box back on. I don't see any way of getting that to be maintained when HandBrake starts a new transcode.